Sondas FISH HealthCare

IBIAN Technologies es distribuidor oficial de la empresa HealthCare en España.

HealthCare Biotecnología ofrece toda una serie de productos para el diagnóstico clínico molecular que se utilizan principalmente en enfermedades cardiovasculares, cáncer y otras enfermedades importantes.
Los principales productos son:

Sondas FISH para:

  • Tumores sólidos
  • Enfermedades relacionados con el sistema hematopoyético linfático
  • Diagnóstico prenatal y examen postnatal
  • Sondas FISH personalizadas

PRUEBAS FARMACOGENETICAS

  • Cardiovascular y Cerebrovascular
  • Metabolismo humano

Con la filosofía empresarial de “Innovación Tecnológica e Innovación de Modelo de Negocio”, HealthCare Biotecnología colabora ampliamente con hospitales, proveedores reconocidos internacionalmente e instituciones de investigación. La compañía adopta un programa de I + D integrado para mejorar la eficiencia de sus productos, la tecnología de productos y la satisfacción del cliente.

Productos FISH:

Ready For use FISH Probe Kits 

By Fluorescence In Situ Hybridization Method (FISH) 

Test ItemCat#Product NameProbe NameSize
 Non-Small Cell Lung CancerKL-LU001Human ALK gene fusion detection probeALK100μL
KL-LU0026q probe (6q22)ROS1100μL
KL-LU003C-MET gene detection probeC-MET/CEP7100μL
Breast CancerKL-BR001Human HER-2 gene amplification detection probeHER2/CEP17100μL
Stomach CancerKL-ST001Human HER-2 gene amplification detection probeHER2100μL
 Bladder CancerKL-BL001  Bladder Cancer cells chromosome and gene anomaly detection probeCEP3/CEP7100μL
KL-BL002P16/CEP17100μL
KL-BL003P53 gene probeP53/CEP17100μL
Cervical CancerKL-CE001TERC gene amplification detection probeTER/CEP3100μL
Brain TumorKL-BT001BRAF gene probe (KIAA1549/BRAF)BRAF100μL
KL-BT0031p/19q chromosome deletion probe1p/19q100μL
 


  Lymphoma		KL-LY001BCL6 gene break apart rearrangement probeBCL6100μL
KL-LY002BCL2 gene break apart rearrangement probeBCL2100μL
KL-LY003IGH gene break apart rearrangement probeIGH100μL
KL-LY004MYC/IGH t (8;14) gene fusion probeIGH/C-MYC100μL
KL-LY005BCL2/IGH t (14;18) gene fusion probeIGH/BCL2100μL
KL-LY006P53 gene probeP53/CEP17100μL
KL-LY007MYC gene break apart rearrangement probeMYC100μL
KL-LY008CCND1 (BCL1)/IGH gene fusion probeCCND1/IGH100μL

  Chronic Lymphocytic Leukemia (CLL)		KL-CLL01 CLL chromosome and gene anomaly detection probeRB1/ATM100μL
KL-CLL02P53/CEP17100μL
KL-CLL03D13S319/CEP12100μL
KL-CLL04Chromosome 12 centromeric probeCEP12100μL
KL-CLL05ATM gene deletion probeCEP11/ATM100μL
KL-CLL0613(13q14)/13q34 probeD13S319/LAMP1 (13q34)100μL
KL-CLL07MYB (6q23) gene probeMYB/CEP6100μL
 

Acute Myeloid Leukemia /AML (Non APL)		KL-AML01RUNX1/RUNX1T1 t (8;21) gene fusion probeAML1/ETO100μL
KL-AML02PML/RARA gene fusion probePML/RARA100μL
KL-AML03CBFB gene break apart rearrangement detection probeCBFB100μL
KL-AML04KMT2A (MLL) gene break apart rearrangement detection probeMLL100μL
KL-AML05CBFB/MYH11 gene fusion probeCBFB/MYH11100μL
Chronic Myeloid Leukemia(CML) KL-CML01 BCR/ABL gene fusion detection probe BCR/ABL 100μL
 



  Acute Lymphoblastic Leukemia (ALL)		KL-ALL01ETV6 (TEL)/RUNX1 (AML1) gene translocation probeTEL/AML1100μL
KL-ALL02BCR/ABL gene fusion detection probeBCR/ABL100μL
KL-ALL03IGH gene break apart rearrangement probeIGH100μL
KL-ALL04P16 gene deletion probeP16 (CDK2NA)/CEP9100μL
KL-ALL05KMT2A (MLL) gene break apart rearrangement probeMLL100μL
KL-ALL06Chromosomes 4, 10 probeCEP4/CEP10100μL
KL-ALL07Chromosome 17 centromeric probeCEP17100μL
KL-ALL08MYC gene break apart rearrangement probeMYC100μL
KL-ALL09TCF3/PBX1gene fusion probeTCF3/PBX1100μL
 
 Multiple Myeloma(MM)		KL-MM001RB1 gene deletion probeRB1100μL
KL-MM0021q21 gene probe1q21100μL
KL-MM003P53 gene probeP53/CEP17100μL
KL-MM004IGH gene break apart probeIGH100μL
KL-MM005CCND1 (BCL1)/IGH gene fusion probeCCND1/IGH100μL
KL-MM00613(13q14)/13q34 probeD13S319/LAMP1(13q34)100μL
 


Myelodysplastic Syndrome(MDS)   		KL-MDS01 
  MDS chromosome and gene anomaly detection probe  		D7S486/CEP7100μL
KL-MDS02D7S522/CEP7100μL
KL-MDS03CSF1R/D5S630100μL
KL-MDS04EGR1/D5S630 5P15(D5S630)/5q31.2(EGR1)100μL
KL-MDS05D20S108/CEP8100μL
KL-MDS06CEPY/CEPX100μL
KL-MDS07Chromosome 8 centromeric probeCEP8100μL
  Aplastic AnemiaKL-AN01Chromosome 8 centromeric probeCEP8100μL
KL-AN027q chromosome deletion [del(7q)] probeD7S486/CEP7100μL
KL-AN035q chromosome deletion [del(5q)] probeEGR1/D5S630 5P15(D5S630)/5q31.2(EGR1)100μL
KL-AN0420q chromosome deletion [del(20q)] probe20q12(D20S108)/20q13.12100μL
Flexible Dye Selection
Fluorescein colorExcitation wavelengthEmission wavelengthFilter
Orange (RHO)553565Orange Monochannel ; Green/Orange Dual Channel
Green (FITC)495518Green Monochannel ; Green/Orange Dual Channel
DAPI (Blue)367452Monochannel

Our Advantages:

a). FISH probes (FastProbe®) hybridization time of only 2 hours (cells and tissue) against 16-24 hours for similar products in the market. 

b). FISH probes (FastProbe®) using the Non-Repetitive Sequences technology ensuing a product with a very good hybridization result, good and clear signal to background noise, high specificity and accuracy.

c). FISH probes (FastProbe®) with a very good anti-quenching effect allowing long observation under fluorescence microscope.

d). An excellent customer service with very short lead time from PO confirmation to shipment.

e). Very competitive affordable price. 

Fast Fluorescence In-Situ Hybridization (FISH) Probe

Protocol Manual

        1. SAMPLE

        A. Tissue

        (1) a). Paraffin-embedded tissue samples fixation with 4% formaldehyde for 6-72 hours.

           b). Thickness: 3-5µm

        (2) Glass slides: Adhesive slides.

        B. Cells

        (1) a). Anticoagulated fresh bone marrow (Storage conditions 4oC, within 24h).

           b). Bone marrow cells accurate fixation (Storage conditions -20oC, within 6 months).

        (2) Glass slides: Ordinary/Common glass slides.

        2. SAMPLE PRETREATMENT 

        Pretreatment procedure

        A. Tissue – Slides pretreatment (sample releasing agent)

        1. Slides heating: Heat at 80oC for 30min or 65oC for 2h or overnight.

        2. Dewaxing: Deparaffinization agent 68oC for 15min.

        3. Washing: Wash with anhydrous EtOH at room temperature for 5min.

        4. Permeation: Permeation agent 90oC for 20min or deionized water 90oC for 40min.

        5. Washing: Wash with distilled water at 37oC for 3min.

        6. Digestion: Enzymic digestion at 37oC for 10-40min.

        7. Washing: At room temperature, wash with cleaning solution twice, 5min each time.

        8. Dehydration: With 70%, 85% and 100% gradient EtOH for 2min.

        9. Dry at room temperature.

        B. Cell – Slides pretreatment

        1. Slides heating: Heat at 56oC for 30min.

        2. Washing: At room temperature, wash with cleaning solution twice, 5min each time.

        3. Dehydration: With 70%, 85% and 100% gradient EtOH for 2min.

        4. Dry at room temperature.

        3. DENATURATION & HYBRIDIZATION

        Denaturation

        Before probe use, apply a vortex shock: 5sec.

        A. Tissue – Slides FISH hybridization

        a). 85oC denaturation for 5min.

        b). 42oC hybridization for 2h (for pretreatment made with HealthCare Biotech FISH Pretreatment Reagent Kit) or Overnight (for pretreatment made without HealthCare Biotech FISH Pretreatment Kit Reagent).

        B. Cells – FISH hybridization

        a). 88oC denaturation for 2min.

        b). 45oC hybridization for 2h.

        4. WASHING

        1. Wash at room temperature with 2xSSC solution for 1min.

        2. Washing solution 68oC (Neutral 0.3% NP-40/0.4 x SSC solution) for 3min.

        3. Deionized water at 37oC for 1min.

        4. Dry at room temperature.

        5. DYEING

        DAPI counterstaining for 10 min.

        6. RESULTS READING

        Target area analysis under fluorescence microscope (HealthCare Biotech FastProbe® filter parameters: Green (495/518), Orange (553/565), DAPI (367/452).

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